Neurofilament L Rabbit Polyclonal Antibody (CAB0257)
The NEFL Polyclonal Antibody (CAB0257) is a valuable tool for researchers studying neurofilament proteins and their role in neurological disorders. Neurofilaments are structural proteins found in neurons, and abnormalities in these proteins have been linked to various neurological conditions such as Alzheimer's disease, ALS, and neuropathies.This antibody, developed using rabbits as the host animal, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically targeting the NEFL protein, researchers can detect and analyze neurofilament expression in a variety of cell types, aiding in the investigation of neurodegenerative diseases and potential therapeutic targets.
Given the importance of neurofilament proteins in maintaining neuronal structure and function, understanding their regulation and dysregulation is crucial for advancing our knowledge of neurological disorders. The NEFL Polyclonal Antibody offers a reliable tool for researchers seeking insights into the role of neurofilaments in health and disease.
Product Name:
Neurofilament L Rabbit Polyclonal Antibody
SKU:
CAB0257
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2).
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Purification Method:
Affinity purification
Gene ID:
4747
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Neurofilament L Rabbit pAb (CAB0257) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
