Anti-NEDD8 Antibody (CAB13520)
- SKU:
- CAB13520
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
Anti-NEDD8 Antibody (CAB13520)
The NEDD8 Polyclonal Antibody (CAB13520) is a valuable tool for researchers studying the NEDD8 protein, a key player in the process of neddylation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the NEDD8 protein, researchers can detect and analyze its expression in various cell types, providing insights into cellular processes such as protein degradation, cell cycle regulation, and DNA damage response.NEDD8 is a ubiquitin-like protein modifier that plays a crucial role in regulating the activity of Cullin-RING ubiquitin ligases (CRLs), which are involved in the ubiquitination and degradation of target proteins.
Dysregulation of neddylation has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Therefore, studying the function of NEDD8 and its interactions with CRLs is essential for developing targeted therapies and understanding the underlying mechanisms of these diseases.Researchers in the fields of cell biology, biochemistry, and oncology can benefit from the use of the NEDD8 Polyclonal Antibody in their studies. Its specificity and reliability make it an indispensable tool for investigating the intricate network of protein modifications and their impact on cellular processes.
| Antibody Name: | Anti-NEDD8 Antibody |
| Antibody SKU: | CAB13520 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLIK VKTL TGKE IEID IEPT DKVE RIKE RVEE KEGI PPQQ QRLI YSGK QMND EKTA ADYK ILGG SVLH LVLA LRGG GGLR Q |
| Gene ID: | 4738 |
| Uniprot: | Q15843 |
| Cellular Location: | Nucleus |
| Calculated MW: | 9kDa |
| Observed MW: | 13kDa |
| Synonyms: | NEDD8, NEDD-8 |
| Background: |
| UniProt Protein Function: | NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain. |
| UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 14q12 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; protein localization; anatomical structure morphogenesis; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance |
| UniProt Code: | Q15843 |
| NCBI GenInfo Identifier: | 2833270 |
| NCBI Gene ID: | 4738 |
| NCBI Accession: | Q15843.1 |
| UniProt Secondary Accession: | Q15843,Q3SXN8, Q6LES6, |
| UniProt Related Accession: | Q15843 |
| Molecular Weight: | 81 |
| NCBI Full Name: | NEDD8 |
| NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 8 |
| NCBI Official Symbol: | NEDD8Â Â |
| NCBI Official Synonym Symbols: | NEDD-8Â Â |
| NCBI Protein Information: | NEDD8; neddylin; ubiquitin-like protein Nedd8 |
| UniProt Protein Name: | NEDD8 |
| UniProt Synonym Protein Names: | Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 |
| Protein Family: | NEDD8 |
| UniProt Gene Name: | NEDD8Â Â |
| UniProt Entry Name: | NEDD8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEDD8 antibody (CAB13520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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