The NEDD8 Monoclonal Antibody (CAB22568) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ubiquitin protein ligase binding activity. Acts upstream of or within protein neddylation. Located in cytosol and nucleoplasm. Biomarker of Parkinson's disease and malignant astrocytoma. RRID Gene ID 4738 Swiss Prot Synonym NEDD-8; NEDD8
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEDD8 Monoclonal Antibody
SKU:
CAB22568
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC58460
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NEDD-8, NEDD8
Positive Sample:
293T, RAW264.7, NIH/3T3, C6, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
9kDa
Observed MW:
9kDa
Enables ubiquitin protein ligase binding activity. Acts upstream of or within protein neddylation. Located in cytosol and nucleoplasm. Biomarker of Parkinson's disease and malignant astrocytoma. RRID Gene ID 4738 Swiss Prot Synonym NEDD-8; NEDD8
Purification Method:
Affinity purification
Gene ID:
4738
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NEDD8 Rabbit mAb (CAB22568) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
