The NEDD4L Polyclonal Antibody (CAB8085) is a valuable tool for researchers studying NEDD4L, a crucial regulator of cell growth and proliferation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.NEDD4L, a member of the NEDD4 family of E3 ubiquitin ligases, plays a key role in the ubiquitination and degradation of target proteins involved in cell signaling pathways. Dysregulation of NEDD4L has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.
By binding to NEDD4L, this polyclonal antibody allows for the detection and analysis of NEDD4L levels in different cell types, providing valuable insights into its function and potential therapeutic targets. Researchers in the fields of oncology, cardiovascular biology, and neurology will find this antibody essential for their studies on NEDD4L and its impact on disease pathology.
Product Name:
NEDD4L Rabbit Polyclonal Antibody
SKU:
CAB8085
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
23327
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NEDD4L Rabbit pAb (CAB8085) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
