The NEDD4 Rabbit Monoclonal Antibody (CAB4385) is a powerful tool for researchers studying the NEDD4 protein, a key regulator of protein degradation and turnover in cells. This antibody, produced using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting NEDD4 in human samples, making it an ideal choice for Western blot assays. By targeting NEDD4, researchers can investigate its role in various cellular processes, including protein ubiquitination and degradation, and its potential implications in diseases such as cancer and neurological disorders.
NEDD4, a member of the NEDD4 family of E3 ubiquitin ligases, plays a crucial role in the regulation of protein stability and turnover by tagging proteins with ubiquitin for degradation by the proteasome. Dysregulation of NEDD4 has been linked to various diseases, making it a promising therapeutic target for drug development and precision medicine approaches. By utilizing the NEDD4 Rabbit Monoclonal Antibody (CAB4385), researchers can gain valuable insights into the molecular mechanisms governed by NEDD4 and explore its therapeutic potential in treating NEDD4-related disorders.
Product Name:
NEDD4 Rabbit Monoclonal Antibody
SKU:
CAB4385
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human NEDD4 (P46934).
This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.
Purification Method:
Affinity purification
Gene ID:
4734
Clone Number:
ARC0992
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit mAb (CAB4385) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
