The NEDD4 Polyclonal Antibody (CAB0552) is a high-quality antibody designed for research involving NEDD4, a key protein in the regulation of various cellular processes. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding specifically to NEDD4, this antibody allows for accurate detection and analysis of the protein in a variety of cell types, making it an ideal tool for studies in cell biology and molecular research.NEDD4, also known as neural precursor cell expressed developmentally down-regulated 4, is a HECT-type E3 ubiquitin ligase that plays a crucial role in the regulation of protein degradation and signaling pathways.
Its involvement in various cellular functions, such as cell proliferation, apoptosis, and receptor internalization, makes it a valuable target for investigation in research areas like cancer, neurodegenerative diseases, and developmental biology. Understanding the functions and regulatory mechanisms of NEDD4 is essential for advancing our knowledge of these complex biological processes and developing potential therapeutic strategies.
Product Name:
NEDD4 Rabbit Polyclonal Antibody
SKU:
CAB0552
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2).
This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.
Purification Method:
Affinity purification
Gene ID:
4734
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NEDD4 Rabbit pAb (CAB0552) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
