The NDUFAF1 Polyclonal Antibody (CAB8014) is a valuable tool for researchers studying NDUFAF1, a protein involved in mitochondrial function and respiratory chain assembly. This antibody, generated in rabbits, demonstrates high specificity for human samples and is optimized for use in Western blot analysis. By binding to NDUFAF1, it enables precise detection and analysis of this protein in a variety of cell types, making it ideal for investigations in mitochondrial biology and metabolic diseases.NDUFAF1 is a crucial factor in the assembly of complex I of the mitochondrial respiratory chain, essential for ATP production and cellular energy metabolism.
Dysregulation of NDUFAF1 has been linked to various human diseases, including mitochondrial disorders and neurodegenerative conditions. By studying the role of NDUFAF1, researchers can gain insights into the mechanisms underlying these disorders and potentially identify new therapeutic targets for intervention. The NDUFAF1 Polyclonal Antibody offers a valuable tool for advancing our understanding of mitochondrial function and its implications in health and disease.
Product Name:
NDUFV1 Rabbit Polyclonal Antibody
SKU:
CAB8014
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 72-420 of human NDUFV1 (NP_009034.2).
The mitochondrial respiratory chain provides energy to cells via oxidative phosphorylation and consists of four membrane-bound electron-transporting protein complexes (I-IV) and an ATP synthase (complex V). This gene encodes a 51 kDa subunit of the NADH:ubiquinone oxidoreductase complex I; a large complex with at least 45 nuclear and mitochondrial encoded subunits that liberates electrons from NADH and channels them to ubiquinone. This subunit carries the NADH-binding site as well as flavin mononucleotide (FMN)- and Fe-S-biding sites. Defects in complex I are a common cause of mitochondrial dysfunction; a syndrome that occurs in approximately 1 in 10,000 live births. Mitochondrial complex I deficiency is linked to myopathies, encephalomyopathies, and neurodegenerative disorders such as Parkinson's disease and Leigh syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
4723
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFV1 Rabbit pAb (CAB8014) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
