The NDUF4 Polyclonal Antibody (CAB6390) is a valuable tool for researchers studying NDUF4, a key subunit of the mitochondrial membrane respiratory chain complex I. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the NDUF4 protein, this antibody enables researchers to detect and analyze NDUF4 expression in various cell types, making it an essential tool for studies in mitochondrial biology, metabolism, and energy production.
NDUF4 is a critical component of complex I, which plays a central role in the electron transport chain and ATP synthesis in the mitochondria. Dysregulation of complex I function has been linked to a variety of diseases, including neurodegenerative disorders and metabolic diseases. Studying NDUF4 expression and activity can provide important insights into mitochondrial function and its impact on cellular processes, making this antibody an invaluable resource for research into mitochondrial dysfunction and related diseases.
Product Name:
NDUFS4 Rabbit Polyclonal Antibody
SKU:
CAB6390
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human NDUFS4 (NP_002486.1).
This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4724
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain, using NDUFS4 Rabbit pAb (CAB6390) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
