The NDUFS4 Polyclonal Antibody (CAB13519) is a valuable tool for research involving NDUFS4, a subunit of the mitochondrial complex I enzyme involved in oxidative phosphorylation. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the NDUFS4 protein, enabling accurate detection and analysis in various cell types, making it an excellent choice for studies in mitochondrial biology and metabolic diseases.NDUFS4 is essential for the function of complex I, the largest and most complicated enzyme of the mitochondrial electron transport chain.
Mutations in NDUFS4 have been linked to mitochondrial diseases, including Leigh syndrome and mitochondrial complex I deficiency. Understanding the role of NDUFS4 in mitochondrial function is crucial for developing targeted therapies to treat these diseases and improve patient outcomes. Overall, the NDUFS4 Polyclonal Antibody is a reliable tool for researchers studying mitochondrial complex I function, oxidative phosphorylation, and related metabolic disorders. Its high specificity and sensitivity make it well-suited for a wide range of experiments aimed at elucidating the role of NDUFS4 in health and disease.
Product Name:
NDUFS4 Rabbit Polyclonal Antibody
SKU:
CAB13519
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human NDUFS4 (NP_002486.1).
This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4724
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFS4 Rabbit pAb (CAB13519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
