The NDUFS3 Polyclonal Antibody (AB100011) is a valuable tool for researchers studying NDUFS3, a subunit of the mitochondrial complex I involved in cellular respiration. This antibody, produced in rabbits, specifically targets human NDUFS3 and is validated for use in Western blot applications. By binding to NDUFS3, researchers can detect and analyze this important protein in various cell types, making it ideal for studies in bioenergetics, metabolism, and mitochondrial disorders.NDUFS3 is essential for the function of complex I, the largest enzyme complex in the electron transport chain responsible for generating ATP.
Malfunction of NDUFS3 has been linked to various mitochondrial diseases, including Leigh syndrome and mitochondrial encephalopathy. Understanding the role of NDUFS3 is crucial for unraveling the mechanisms of these disorders and developing potential treatments. Researchers studying mitochondrial function and bioenergetics will find the NDUFS3 Polyclonal Antibody to be a valuable asset in their investigations.
Product Name:
NDUFS3 Polyclonal Antibody
SKU:
CAB3983
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
Purification Method:
Affinity purification
Gene ID:
4722
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of THP-1 cells, using NDUFS3 antibody (CAB3983).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
