The NDUFB5 Polyclonal Antibody (CAB8327) is a valuable tool for researchers studying NDUFB5, a subunit of the mitochondrial membrane respiratory chain complex I. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in immunohistochemistry and Western blot applications. By targeting the NDUFB5 protein, this antibody allows for the precise detection and analysis of mitochondrial function in various cell types, making it ideal for studies in bioenergetics and mitochondrial dysfunction.NDUFB5 is a crucial component of complex I, the largest enzyme complex in the mitochondrial electron transport chain.
As such, it plays a vital role in the generation of cellular energy through oxidative phosphorylation. Dysregulation of complex I function, including NDUFB5, has been implicated in a variety of human diseases, including neurodegenerative disorders and metabolic conditions. Investigating the role of NDUFB5 can provide important insights into the mechanisms underlying these pathologies and may lead to the development of novel therapeutics targeting mitochondrial dysfunction.
Product Name:
NDUFB5 Rabbit Polyclonal Antibody
SKU:
CAB8327
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1).
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4711
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFB5 Rabbit pAb (CAB8327) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
