The NDUFB5 Polyclonal Antibody (CAB14752) is a valuable tool for researchers studying NDUFB5, a subunit of mitochondrial complex I that plays a crucial role in electron transport and ATP production. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.NDUFB5 is essential for the functioning of mitochondrial complex I, which is a key player in oxidative phosphorylation and energy production in the cell. Dysregulation of mitochondrial complex I has been linked to various diseases, including neurodegenerative disorders and metabolic diseases.
By targeting NDUFB5, researchers can gain insights into the role of mitochondrial dysfunction in these conditions.With its high reactivity and specificity, the NDUFB5 Polyclonal Antibody is a valuable tool for studying the molecular mechanisms underlying mitochondrial complex I dysfunction and its implications for human health. Researchers in fields such as biochemistry, cell biology, and metabolism will find this antibody indispensable for their research endeavors.
Product Name:
NDUFB5 Rabbit Polyclonal Antibody
SKU:
CAB14752
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1).
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4711
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NDUFB5 antibody (CAB14752) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
