The NDUFB5 Polyclonal Antibody is a valuable tool for researchers studying the NDUFB5 protein, a critical subunit of the mitochondrial respiratory chain complex I. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the NDUFB5 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of bioenergetics, metabolism, and mitochondrial dysfunction.NDUFB5 is essential for the assembly and function of complex I, the largest and most complex enzyme in the respiratory chain responsible for oxidative phosphorylation. Dysregulation of complex I activity, often associated with mutations in NDUFB5, can lead to mitochondrial dysfunction and various metabolic disorders.
Therefore, studying the role of NDUFB5 is crucial for understanding the mechanisms underlying mitochondrial diseases and developing targeted therapies to address these conditions.By utilizing the NDUFB5 Polyclonal Antibody in your research, you can gain valuable insights into the function of this key mitochondrial protein and its implications for cellular metabolism and energy production. Whether investigating mitochondrial disorders, metabolic syndromes, or neurodegenerative diseases, this antibody can aid in advancing scientific knowledge and potentially identifying novel therapeutic targets for treating mitochondrial dysfunction-related pathologies.
Product Name:
NDUFB5 Polyclonal Antibody
SKU:
CAB21503
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1).
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4711
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NDUFB5 antibody (CAB21503) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
