The NDUFAF4 Polyclonal Antibody (CAB14345) is a valuable tool for research targeting NDUFAF4, a protein involved in mitochondrial respiratory chain complex I assembly. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the NDUFAF4 protein, researchers can detect and analyze its expression in various cell types, making it ideal for studies in mitochondrial biology and related fields.NDUFAF4 is essential for the proper assembly and functioning of complex I in the mitochondrial respiratory chain, which plays a crucial role in cellular energy production.
Dysregulation of complex I assembly has been linked to various diseases, including mitochondrial disorders and neurodegenerative conditions. Investigating the role of NDUFAF4 can provide insights into the mechanisms of these diseases and potentially lead to the development of targeted therapies aimed at restoring mitochondrial function.
Product Name:
NDUFAF4 Rabbit Polyclonal Antibody
SKU:
CAB14345
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NDUFAF4 (NP_054884.1).
NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.
Purification Method:
Affinity purification
Gene ID:
29078
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFAF4 Rabbit pAb (CAB14345) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
