The NDUFA5 Polyclonal Antibody (CAB3976) is a valuable tool for researchers studying the NDUFA5 protein, which is a subunit of the mitochondrial respiratory chain Complex I. This antibody, produced in rabbits, is highly specific for human samples and is ideal for use in Western blot applications. By targeting the NDUFA5 protein, this antibody enables accurate detection and analysis in various cell types, making it essential for investigations in areas such as mitochondrial function, energy metabolism, and cellular respiration.The NDUFA5 protein is critical for electron transport and ATP production in the mitochondria, making it a key player in cellular energy metabolism. Dysregulation of NDUFA5 has been linked to various diseases, including mitochondrial disorders and neurodegenerative conditions.
Research into the functions and mechanisms of NDUFA5 is crucial for understanding these disease processes and developing potential therapeutic interventions targeting mitochondrial dysfunction.Overall, the NDUFA5 Polyclonal Antibody (CAB3976) offers researchers a reliable tool for studying the role of NDUFA5 in mitochondrial function and its implications for health and disease. Its high specificity and reactivity with human samples make it a valuable asset for advancing our understanding of mitochondrial biology and potential therapeutic targets.
Product Name:
NDUFA5 Rabbit Polyclonal Antibody
SKU:
CAB3976
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
Purification Method:
Affinity purification
Gene ID:
4698
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NDUFA5 antibody (CAB3976) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
