The NDUFA5 Polyclonal Antibody (CAB14751) is a valuable tool for researchers studying NDUFA5, a critical subunit of the mitochondrial respiratory chain complex I. This antibody, raised in rabbits, is highly specific to human NDUFA5 protein and is validated for use in Western blotting applications. By binding to NDUFA5, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in mitochondrial function and energy metabolism.NDUFA5 is essential for the proper function of complex I in the electron transport chain, playing a key role in ATP production and cellular respiration.
Dysregulation of NDUFA5 has been linked to various mitochondrial disorders and diseases, making it a target of interest in research on neurodegenerative disorders, metabolic diseases, and aging. Understanding the function and regulation of NDUFA5 is crucial for advancing our knowledge of mitochondrial biology and developing potential therapeutic interventions.
Product Name:
[KO Validated] NDUFA5 Rabbit Polyclonal Antibody
SKU:
CAB14751
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
Purification Method:
Affinity purification
Gene ID:
4698
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFA5 Rabbit pAb (CAB14751) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
