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NDUFA4 Polyclonal Antibody (CAB21502)

SKU:
CAB21502
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
IF
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:NDUFA4 Polyclonal Antibody
SKU:CAB21502
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NDUFA4 (NP_002480.1).
Sequence:MLRQ IIGQ AKKH PSLI PLFV FIGT GATG ATLY LLRL ALFN PDVC WDRN NPEP WNKL GPND QYKF YSVN VDYS KLKK ERPD F
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:MLRQ; CI-9k; COXFA4; MISTR1; MRCAF1; CI-MLRQ; MC4DN21; A4
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein.
Calculated MW:9kDa
Observed MW:10kDa

The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Purification Method:Affinity purification
Gene ID:4697
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type(WT) and NDUFA4 knockout (KO) 293T(KO) cells, using [KO Validated] NDUFA4 Rabbit pAb (CAB21502) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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