The NDUFA4 Polyclonal Antibody (CAB15693) is a valuable tool for researchers studying the NDUFA4 protein, which is a subunit of the mitochondrial respiratory chain complex I. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for Western blot applications.The NDUFA4 protein plays a crucial role in electron transport and ATP production within the mitochondria, making it essential for cellular energy metabolism. Dysregulation of NDUFA4 has been linked to various diseases, including mitochondrial disorders and neurodegenerative conditions.
By targeting the NDUFA4 protein, researchers can gain insights into mitochondrial function and its impact on cellular processes. The antibody's specificity and sensitivity make it an excellent tool for investigating the role of NDUFA4 in health and disease, providing valuable information for studies in mitochondrial biology, metabolism, and related fields.
Product Name:
NDUFA4 Rabbit Polyclonal Antibody
SKU:
CAB15693
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NDUFA4 (NP_002480.1).
The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Purification Method:
Affinity purification
Gene ID:
4697
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251 MG cells using NDUFA4 Rabbit pAb (CAB15693) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
