NDN Antibody (PACO58440)
- SKU:
- PACO58440
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
NDN Antibody (PACO58440)
The NDN Antibody (PACO58440) is a polyclonal antibody designed for research involving the NDN protein, a crucial regulator of immune function and neurological development. The antibody, raised in rabbits, is highly specific and validated for use in various applications, including immunofluorescence and immunohistochemistry.NDN, also known as Nestin gene downstream, plays a key role in the development and function of the central nervous system, making it a vital target for research in neurobiology and neuroscience. Its involvement in neurological disorders such as autism and intellectual disability highlights its potential as a biomarker and therapeutic target for these conditions.
The NDN Antibody enables the detection and analysis of NDN protein expression in different cell types and tissues, providing researchers with valuable insights into its functions and mechanisms of action. Its versatility and reliability make it an essential tool for studying the role of NDN in health and disease, paving the way for new discoveries and potential therapeutic interventions.
| Antibody Name: | NDN Antibody (PACO58440) |
| Antibody SKU: | PACO58440 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Necdin protein (1-98AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58440 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO58440 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity). |
| Synonyms: | Necdin, NDN |
| UniProt Protein Function: | NDN: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 15q11.2-q12 Biological Process: nervous system development Disease: Prader-willi Syndrome |
| NCBI Summary: | This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99608 |
| NCBI GenInfo Identifier: | 17380142 |
| NCBI Gene ID: | 4692 |
| NCBI Accession: | Q99608.1 |
| UniProt Secondary Accession: | Q99608,B2R6Z5, |
| UniProt Related Accession: | Q99608 |
| Molecular Weight: | 36,086 Da |
| NCBI Full Name: | Necdin |
| NCBI Synonym Full Names: | necdin, MAGE family member |
| NCBI Official Symbol: | NDNÂ Â |
| NCBI Official Synonym Symbols: | PWCR; HsT16328Â Â |
| NCBI Protein Information: | necdin |
| UniProt Protein Name: | Necdin |
| Protein Family: | Necdin |
| UniProt Gene Name: | NDNÂ Â |
| UniProt Entry Name: | NECD_HUMAN |
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