NDFIP1 Antibody (PACO42094)
- SKU:
- PACO42094
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
NDFIP1 Antibody (PACO42094)
The NDFIP1 Polyclonal Antibody (PAC042094) is a valuable tool for researchers studying NDFIP1, a key regulator of protein degradation and cellular signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot and immunohistochemistry applications. By specifically binding to the NDFIP1 protein, this antibody enables the detection and analysis of NDFIP1 expression in various cell types, making it ideal for investigations in cell biology and signal transduction research.NDFIP1, also known as NEDD4 family interacting protein 1, plays a critical role in the regulation of ubiquitin-mediated protein degradation and the modulation of various signaling cascades.
Its involvement in these processes highlights its importance in cellular homeostasis and makes it a promising target for research in areas such as cancer biology, neurodegenerative diseases, and metabolic disorders. By elucidating the functions of NDFIP1, researchers can gain valuable insights into disease mechanisms and potentially uncover new therapeutic targets for intervention.
| Antibody Name: | NDFIP1 Antibody (PACO42094) |
| Antibody SKU: | PACO42094 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human NEDD4 family-interacting protein 1 protein (2-116AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: NDFIP1 antibody at 8µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 25, 17 kDa. Observed band size: 25 kDa. |
 | Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO42094 at dilution of 1:100. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using PACO42094 at dilution of 1:100. |
| Background: | Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1. |
| Synonyms: | NEDD4 family-interacting protein 1 (Breast cancer-associated protein SGA-1M) (NEDD4 WW domain-binding protein 5) (Putative MAPK-activating protein PM13) (Putative NF-kappa-B-activating protein 164) (Putative NFKB and MAPK-activating protein), NDFIP1, N4WBP5 |
| UniProt Protein Function: | NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 5q31.3Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortexMolecular Function: protein binding; signal transducer activity; WW domain bindingBiological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; negative regulation of transporter activity; regulation of isotype switching to IgG isotypes |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BT67 |
| NCBI GenInfo Identifier: | 74733098 |
| NCBI Gene ID: | 80762 |
| NCBI Accession: | Q9BT67.1 |
| UniProt Secondary Accession: | Q9BT67,Q658T8, Q8N2E3, Q8N2F9, B2RDB8, D3DQF0 |
| UniProt Related Accession: | Q9BT67 |
| Molecular Weight: | 24,899 Da |
| NCBI Full Name: | NEDD4 family-interacting protein 1 |
| NCBI Synonym Full Names: | Nedd4 family interacting protein 1 |
| NCBI Official Symbol: | NDFIP1Â Â |
| NCBI Official Synonym Symbols: | N4WBP5Â Â |
| NCBI Protein Information: | NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164 |
| UniProt Protein Name: | NEDD4 family-interacting protein 1 |
| UniProt Synonym Protein Names: | Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein |
| Protein Family: | NEDD4 family-interacting protein |
| UniProt Gene Name: | NDFIP1Â Â |
| UniProt Entry Name: | NFIP1_HUMAN |
| Antibodies |
| NDFIP1 Antibody (PACO05403) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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