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NDFIP1 Antibody (PACO22728)

SKU:
PACO22728
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
$503

Description

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NDFIP1 Antibody (PACO22728)

The NDFIP1 Polyclonal Antibody (PAC022728) is a versatile tool for researchers studying NDFIP1, a key regulator of protein degradation in cells. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the NDFIP1 protein, this antibody facilitates the detection and analysis of NDFIP1 in various cell types, making it an essential reagent for studies in molecular biology and cell signaling pathways.NDFIP1, also known as Nedd4 family interacting protein 1, plays a crucial role in the regulation of protein turnover and degradation through the ubiquitin-proteasome system.

Dysregulation of NDFIP1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and immune-related conditions. Investigating the function and mechanisms of NDFIP1 is vital for understanding its role in these pathological processes and developing targeted therapies for related diseases.

Antibody Name:NDFIP1 Antibody (PACO22728)
Antibody SKU:PACO22728
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:Human, Mouse, Rat
Immunogen:Synthesized peptide derived from C-terminal of human NDFIP1.
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from 293 cells, using NDFIP1 antibody.
Background:Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation By similarity. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.
Synonyms:Breast cancer-associated protein SGA-1M; MGC10924; N4WBP5; NDFIP1; Nedd4 family interacting protein 1
UniProt Protein Function:NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 5q31.3Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortexMolecular Function: protein binding; signal transducer activity; WW domain bindingBiological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; negative regulation of transporter activity; regulation of isotype switching to IgG isotypes
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BT67
NCBI GenInfo Identifier:74733098
NCBI Gene ID:80762
NCBI Accession:Q9BT67.1
UniProt Secondary Accession:Q9BT67,Q658T8, Q8N2E3, Q8N2F9, B2RDB8, D3DQF0
UniProt Related Accession:Q9BT67
Molecular Weight:24,899 Da
NCBI Full Name:NEDD4 family-interacting protein 1
NCBI Synonym Full Names:Nedd4 family interacting protein 1
NCBI Official Symbol:NDFIP1  
NCBI Official Synonym Symbols:N4WBP5  
NCBI Protein Information:NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164
UniProt Protein Name:NEDD4 family-interacting protein 1
UniProt Synonym Protein Names:Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein
Protein Family:NEDD4 family-interacting protein
UniProt Gene Name:NDFIP1  
UniProt Entry Name:NFIP1_HUMAN
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