The Ndc80 Polyclonal Antibody (CAB5411) is a valuable tool for researchers studying the Ndc80 protein, a key component of the kinetochore complex essential for proper chromosome segregation during cell division. This antibody, raised in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications.The Ndc80 protein plays a crucial role in mitosis, ensuring accurate chromosome alignment and segregation. Dysregulation of Ndc80 has been linked to chromosomal instability and cancer development, highlighting its significance in cell division and genomic stability.
By targeting the Ndc80 protein, researchers can gain insights into the mechanisms underlying these processes and potential therapeutic targets for cancer treatment.With its proven reactivity and effectiveness in detecting the Ndc80 protein, the CAB5411 antibody is a valuable tool for studies in cell biology, cancer research, and drug development. Its ability to specifically bind to Ndc80 enables precise detection and analysis of this important protein, providing researchers with the means to further explore its role in cellular functions and disease pathology.
Product Name:
HEC1/NDC80 Rabbit Polyclonal Antibody
SKU:
CAB5411
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human HEC1/HEC1/NDC80 (NP_006092.1).
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
Purification Method:
Affinity purification
Gene ID:
10403
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HEC1/HEC1/NDC80 antibody (CAB5411) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
