MT-ND5 Rabbit Polyclonal Antibody (CAB12465)
- SKU:
- CAB12465
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Product Name: | MT-ND5 Rabbit Polyclonal Antibody |
| SKU: | CAB12465 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of mouse MT-ND5 (NP_904338.1). |
| Sequence: | KIIA FSTS SQLG LMMV TLGM NQPH LAFL HICT HAFF KAML FMCS GSII HSLA DEQD IRKM GNIT KIMP FTSS CLVI GSLA LTGM PFLT GFYS KDLI IEAI N |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 |
| Synonyms: | ND5; MT-ND5 |
| Positive Sample: | U-251MG,HeLa,Mouse heart,Rat heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Mitochondrion inner membrane, Multi-pass membrane protein. |
| Calculated MW: | 67kDa |
| Observed MW: | 67kDa |
Contributes to NADH dehydrogenase (ubiquinone) activity. Predicted to be involved in electron transport coupled proton transport; mitochondrial electron transport, NADH to ubiquinone; and mitochondrial respiratory chain complex I assembly. Located in mitochondrion. Is expressed in central nervous system; heart; liver; metanephros; and retina. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; Leigh disease; and MELAS syndrome. Orthologous to human MT-ND5 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5).
| Purification Method: | Affinity purification |
| Gene ID: | 17721 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using MT-ND5 antibody (CAB12465) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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