The NCSTN Polyclonal Antibody (CAB14505) is specially designed for research involving NCSTN, a protein involved in the regulation of the Notch signaling pathway. The antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the NCSTN protein, researchers can detect and analyze its expression in various cell types, making it an excellent tool for studies in molecular biology and neuroscience.NCSTN is a critical component of the gamma-secretase complex, essential for the proteolytic processing of Notch receptors, which play a role in cell differentiation and development.
Dysregulation of the Notch signaling pathway has been implicated in various diseases, including cancer and neurodegenerative disorders. Understanding the function of NCSTN is crucial for unraveling the molecular mechanisms involved in these conditions and may lead to the development of novel therapeutic strategies targeting the Notch pathway.
Product Name:
NCSTN Rabbit Polyclonal Antibody
SKU:
CAB14505
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 429-669 of human NCSTN (NP_056146.1).
Melanosome, Membrane, Single-pass type I membrane protein.
Calculated MW:
78kDa
Observed MW:
110kDa
This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
23385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NCSTN Rabbit pAb (CAB14505) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
