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Anti-NCOA4 Antibody (CAB5695)[KO Validated]

SKU:
CAB5695
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • KO Validated Antibodies 1 Anti-NCOA4 Antibody CAB5695KO Validated
  • KO Validated Antibodies 1 Anti-NCOA4 Antibody CAB5695KO Validated
  • KO Validated Antibodies 1 Anti-NCOA4 Antibody CAB5695KO Validated
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Description

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Anti-NCOA4 Antibody (CAB5695)[KO Validated]

The NCOA4 Polyclonal Antibody (CAB5695) is a valuable tool for researchers studying NCOA4, a protein involved in the regulation of iron metabolism and autophagy. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the NCOA4 protein, this antibody enables the detailed analysis and detection of NCOA4 in various cell types, making it an essential component for studies in the fields of metabolism, autophagy, and cancer research.NCOA4, also known as nuclear receptor coactivator 4, plays a crucial role in the maintenance of iron homeostasis by mediating the degradation of ferritin through autophagy.

Dysregulation of NCOA4 has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance as a therapeutic target. By gaining a better understanding of NCOA4 function, researchers can develop novel treatments for conditions associated with iron metabolism and autophagy dysfunction. The NCOA4 Polyclonal Antibody is a valuable tool that will aid in furthering these important research efforts.

Antibody Name:Anti-NCOA4 Antibody [KO Validated]
Antibody SKU:CAB5695
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 495-614 of human NCOA4 (NP_005428.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A375, HeLa, MCF7, 293T, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 495-614 of human NCOA4 (NP_005428.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SEWL IRPP YKEG SPKE VPGT EDRA GKQK FKSP MNTS WCSF NTAD WVLP GKKM GNLS QLSS GEDK WLLR KKAQ EVLL NSPL QEEH NFPP DHYG LPAV CDLF ACMQ LKVD KEKW LYRT PLQM
Gene ID:8031
Uniprot:Q13772
Cellular Location:
Calculated MW:32kDa/69kDa/71kDa/73kDa
Observed MW:70kDa
Synonyms:NCOA4, ARA70, ELE1, PTC3, RFG
Background:This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.
UniProt Protein Function:NCOA4: Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oncoprotein; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: nucleus

Biological Process: male gonad development

Disease: Thyroid Carcinoma, Papillary

NCBI Summary:This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]
UniProt Code:Q13772
NCBI GenInfo Identifier:8928226
NCBI Gene ID:8031
NCBI Accession:Q13772.1
UniProt Secondary Accession:Q13772,Q14239, A8K8W5, B4E260, E9PAV7, J3KQN8,
UniProt Related Accession:Q13772
Molecular Weight:73,649 Da
NCBI Full Name:Nuclear receptor coactivator 4
NCBI Synonym Full Names:nuclear receptor coactivator 4
NCBI Official Symbol:NCOA4  
NCBI Official Synonym Symbols:RFG; ELE1; PTC3; ARA70  
NCBI Protein Information:nuclear receptor coactivator 4
UniProt Protein Name:Nuclear receptor coactivator 4
UniProt Synonym Protein Names:Androgen receptor coactivator 70 kDa protein; 70 kDa AR-activator; 70 kDa androgen receptor coactivator; Androgen receptor-associated protein of 70 kDa; Ret-activating protein ELE1
Protein Family:Nuclear receptor coactivator
UniProt Gene Name:NCOA4  
UniProt Entry Name:NCOA4_HUMAN
Anti-NCOA4 Antibody [KO Validated] (CAB5695)Western blot analysis of extracts of various cell lines, using NCOA4 antibody (CAB5695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-NCOA4 Antibody [KO Validated] (CAB5695)Western blot analysis of extracts from normal (control) and NCOA4 knockout (KO) HeLa cells, using NCOA4 antibody (CAB5695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-NCOA4 Antibody [KO Validated] (CAB5695)Immunohistochemistry of paraffin-embedded human lung cancer using NCOA4 antibody (CAB5695) at dilution of 1:100 (40x lens).

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