The NCKAP1 Polyclonal Antibody (CAB12229) is a valuable tool for researchers studying the NCKAP1 protein, a key component of the N-WASP-Arp2/3 complex involved in actin cytoskeleton regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the NCKAP1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies in cell biology and cytoskeletal dynamics.NCKAP1 is essential for cellular processes such as cell migration, cell adhesion, and cytoskeletal rearrangement, making it a crucial target for investigation in developmental biology, cancer research, and neurobiology.
Understanding the role of NCKAP1 in these processes can provide valuable insights into disease mechanisms and potential therapeutic strategies. Researchers can utilize this antibody to explore the functions and interactions of NCKAP1 in different cellular contexts, advancing our understanding of cell physiology and disease pathogenesis.
Product Name:
NCKAP1 Rabbit Polyclonal Antibody
SKU:
CAB12229
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCKAP1 (NP_038464.1).
Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex.
Purification Method:
Affinity purification
Gene ID:
10787
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NCKAP1 Rabbit pAb (CAB12229) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
