The NCK1 Polyclonal Antibody (CAB0999) is a valuable tool for researchers studying NCK1, a signaling protein that plays a key role in cell growth, migration, and development. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the NCK1 protein, this antibody allows for the detection and analysis of NCK1 expression in a variety of cell types, making it an essential component of studies in cell biology and cancer research.NCK1 is a critical mediator of intracellular signaling pathways that regulate cell proliferation and migration. Its involvement in these processes makes it an important target for investigation in diseases such as cancer, where aberrant signaling can contribute to tumor growth and metastasis.
By studying the function of NCK1, researchers can gain insight into potential therapeutic strategies for targeting signaling pathways associated with cancer progression.Overall, the NCK1 Polyclonal Antibody (CAB0999) provides researchers with a powerful tool for investigating the role of NCK1 in cellular processes and disease development. Its specificity and reliability make it an essential reagent for studies aiming to understand the molecular mechanisms underlying cell signaling and potential therapeutic interventions in cancer and other diseases.
Product Name:
NCK1 Rabbit Polyclonal Antibody
SKU:
CAB0999
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 58-377 of human NCK1 (NP_006144.1).
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Purification Method:
Affinity purification
Gene ID:
4690
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using NCK1 Rabbit pAb (CAB0999) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
