The NCF4 Polyclonal Antibody (CAB2096) is a valuable tool for researchers studying the NCF4 protein, a key component in the regulation of immune responses. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been extensively validated for use in Western blot applications. By specifically binding to the NCF4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential reagent for investigations in immunology and cancer research.NCF4, also known as p40phox, is a critical player in the assembly of the phagocyte NADPH oxidase complex, which is essential for the production of reactive oxygen species involved in immune defense and inflammation.
Dysregulation of NCF4 has been linked to immune dysfunction and inflammatory disorders, highlighting its significance as a potential therapeutic target. By unraveling the role of NCF4 in immune regulation, researchers can gain valuable insights that may lead to the development of novel treatments for conditions such as cancer, autoimmune diseases, and chronic inflammatory disorders.
Product Name:
NCF4 Rabbit Polyclonal Antibody
SKU:
CAB2096
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-190 of human NCF4/p40-phox (NP_038202.2).
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
4689
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NCF4/p40-phox antibody (CAB2096) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
