NCF4 Rabbit Polyclonal Antibody (CAB2096)
- SKU:
- CAB2096
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Product Name: | NCF4 Rabbit Polyclonal Antibody |
SKU: | CAB2096 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-190 of human NCF4/p40-phox (NP_038202.2). |
Sequence: | MAVA QQLR AESD FEQL PDDV AISA NIAD IEEK RGFT SHFV FVIE VKTK GGSK YLIY RRYR QFHA LQSK LEER FGPD SKSS ALAC TLPT LPAK VYVG VKQE IAEM RIPA LNAY MKSL LSLP VWVL MDED VRIF FYQS PYDS EQVP QALR RLRP RTRK VKSV SPQG NSVD RMAA PRAE ALFD FTGN SKLE LN |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 |
Synonyms: | NCF; CGD3; P40PHOX; SH3PXD4; NCF4/p40-phox |
Positive Sample: | U-937,RAW264.7,Rat lung |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, cytosol. |
Calculated MW: | 39kDa |
Observed MW: | 40kDa |
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method: | Affinity purification |
Gene ID: | 4689 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |