Anti-NOXA2 / P67phox Antibody (CAB1178)
- SKU:
- CAB1178
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-NOXA2 / P67phox Antibody (CAB1178)
The NCF2 Polyclonal Antibody (CAB1178) is a valuable tool for researchers studying NCF2, a crucial protein involved in the regulation of the NADPH oxidase complex. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the NCF2 protein, this antibody allows for the detection and analysis of NCF2 in various cell types, making it ideal for studies in immunology and cancer research.NCF2, also known as neutrophil cytosolic factor 2, plays a key role in the production of reactive oxygen species essential for various immune responses, including phagocytosis and microbial killing.
Dysregulation of NCF2 has been implicated in a range of diseases, from autoimmune disorders to chronic inflammatory conditions. Understanding the function of NCF2 is crucial for developing therapeutic strategies that target the NADPH oxidase complex and modulate immune responses in disease settings. Overall, the NCF2 Polyclonal Antibody (CAB1178) offers researchers a reliable tool for investigating the role of NCF2 in immune function and disease pathology, ultimately paving the way for the development of novel therapies targeting the NADPH oxidase complex.
| Antibody Name: | Anti-NOXA2 / P67phox Antibody |
| Antibody SKU: | CAB1178 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2 / P67phox (NP_000424.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | THP-1, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2 / P67phox (NP_000424.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPPR PKTP EIFR ALEG EAHR VLFG FVPE TKEE LQVM PGNI VFVL KKGN DNWA TVMF NGQK GLVP CNYL EPVE LRIH PQQQ PQEE SSPQ SDIP APPS SKAP GRPQ LSPG QKQK EEPK EVKL SVPM PYTL KVHY KYTV VMKT QPGL PYSQ VRDM VSKK LELR LEHT KLSY RPRD SNEL VPLS EDSM KDAW GQVK NYCL TLWC ENTV GDQG FPDE PKES EKAD ANNQ TTEP QLKK GSQV EALF SYEA TQPE DLEF QEGD IILV LSKV NEEW LEGE CKGK VGIF PKVF VEDC ATTD LEST RREV |
| Gene ID: | 4688 |
| Uniprot: | P19878 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 47kDa/50kDa/54kDa/59kDa |
| Observed MW: | 68kDa |
| Synonyms: | NCF2, NCF-2, NOXA2, P67-PHOX, P67PHOX |
| Background: | This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family. |
| UniProt Protein Details: | Protein type:Oxidoreductase Chromosomal Location of Human Ortholog: 1q25 Cellular Component: cytoplasm; acrosome; nucleolus; cytosol; NADPH oxidase complex Molecular Function:protein C-terminus binding; protein binding; electron carrier activity; Rac GTPase binding Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to lipopolysaccharide; cellular response to hormone stimulus; antigen processing and presentation of peptide antigen via MHC class I; response to hyperoxia; positive regulation of neuron apoptosis; response to glucose stimulus; cellular defense response; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; response to activity; vascular endothelial growth factor receptor signaling pathway; response to progesterone stimulus; superoxide release; positive regulation of blood pressure; aging Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii |
| NCBI Summary: | This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P19878 |
| NCBI GenInfo Identifier: | 1346669 |
| NCBI Gene ID: | 4688 |
| NCBI Accession: | P19878.2 |
| UniProt Secondary Accession: | P19878,Q2PP06, Q8NFC7, Q9BV51, B2R6Q1, B4DKQ7, B4DQA7 E9PHJ2, E9PHX3, |
| UniProt Related Accession: | P19878 |
| Molecular Weight: | 50,337 Da |
| NCBI Full Name: | Neutrophil cytosol factor 2 |
| NCBI Synonym Full Names: | neutrophil cytosolic factor 2 |
| NCBI Official Symbol: | NCF2Â Â |
| NCBI Official Synonym Symbols: | NCF-2; NOXA2; P67PHOX; P67-PHOXÂ Â |
| NCBI Protein Information: | neutrophil cytosol factor 2; NADPH oxidase activator 2; 67 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 2; chronic granulomatous disease, autosomal 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) |
| UniProt Protein Name: | Neutrophil cytosol factor 2 |
| UniProt Synonym Protein Names: | 67 kDa neutrophil oxidase factor; NADPH oxidase activator 2; Neutrophil NADPH oxidase factor 2; p67-phox |
| UniProt Gene Name: | NCF2Â Â |
| UniProt Entry Name: | NCF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NOXA2 / P67phox antibody (CAB1178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using NOXA2 / P67phox Rabbit pAb (CAB1178) at dilution of 1:100 (40x lens). |
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