The NBR1 Polyclonal Antibody (CAB3949) is a valuable tool for researchers studying the autophagy receptor NBR1, an important protein involved in the process of autophagy. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.NBR1 plays a crucial role in the selective autophagy pathway by recognizing and delivering ubiquitinated cargo for degradation in the autophagosome. By targeting NBR1 with this antibody, researchers can effectively detect and analyze NBR1 protein levels in various cell types, providing insights into the mechanisms of autophagy regulation.
Given the relevance of autophagy dysregulation in various diseases, including cancer, neurodegenerative disorders, and infectious diseases, understanding the function of NBR1 is essential for developing targeted therapies. The NBR1 Polyclonal Antibody offers a reliable tool for investigating the role of NBR1 in autophagy and its potential implications in disease pathogenesis.
Product Name:
NBR1 Rabbit Polyclonal Antibody
SKU:
CAB3949
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human NBR1 (NP_005890.2).
Cytoplasm, Cytoplasmic vesicle, Lysosome, M line, autophagosome, myofibril, sarcomere.
Calculated MW:
107kDa
Observed MW:
120kDa
The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4077
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using NBR1 Rabbit pAb (CAB3949) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
