The NARFL Polyclonal Antibody (PAC022477) is a valuable tool for researchers studying NARFL, a protein involved in various cellular processes, including mitochondrial function and iron-sulfur cluster biogenesis. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for Western blot applications.NARFL is known for its role in maintaining mitochondrial integrity and function, making it essential for cellular energy production and overall cell viability. Dysregulation of NARFL has been linked to various diseases, including neurodegenerative disorders and metabolic conditions.
Therefore, in-depth research on NARFL is crucial for understanding its impact on health and disease.By utilizing the NARFL Polyclonal Antibody, researchers can investigate the expression levels and localization of NARFL in different cell types and tissues. This antibody provides a reliable tool for studying the function and regulation of NARFL, allowing for advancements in the fields of mitochondrial biology, neurology, and metabolism research.
Antibody Name:
NARFL Antibody (PACO22477)
Antibody SKU:
PACO22477
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human NARFL.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from A549 cells, using NARFL antibody.
Background:
Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
Synonyms:
FLJ21988; Nuclear prelamin A recognition factor- like; PRN; Protein related to Narf;
UniProt Protein Function:
NARFL: Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. Belongs to the NARF family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Unknown function
Chromosomal Location of Human Ortholog: 16p13.3
Biological Process: iron-sulfur cluster assembly; oxygen homeostasis; regulation of transcription, DNA-dependent; response to hypoxia
