The NAPRT Polyclonal Antibody (CAB11591) is a valuable tool for researchers studying Nicotinate phosphoribosyltransferase (NAPRT), an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis. This polyclonal antibody, produced in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications.NAPRT plays a crucial role in the NAD salvage pathway, which is essential for cellular metabolism and DNA repair processes. Dysregulation of NAPRT has been linked to various diseases, including cancer and neurodegenerative disorders.
Therefore, understanding the expression and activity of NAPRT is important for elucidating its role in normal physiology and disease states.With its high reactivity and specificity, the NAPRT Polyclonal Antibody is an excellent tool for detecting and quantifying NAPRT levels in different cell types and tissues. Researchers in the field of metabolism, cancer biology, and drug development will find this antibody invaluable for their studies on NAPRT and NAD metabolism pathways.
Product Name:
NAPRT Rabbit Polyclonal Antibody
SKU:
CAB11591
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 309-538 of human NAPRT1 (NP_660202.3).
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).
Purification Method:
Affinity purification
Gene ID:
93100
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NAPRT Rabbit pAb (CAB11591) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
