The NANS Polyclonal Antibody (CAB4778) is a valuable tool for researchers studying NANS, an enzyme involved in sialic acid metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the NANS protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in glycobiology and metabolic research.NANS plays a crucial role in the biosynthesis of sialic acid, a key component of cell surface molecules involved in various biological processes, including cell-cell interactions and signaling.
Dysregulation of sialic acid metabolism has been implicated in a range of human diseases, making NANS a promising target for therapeutic intervention. By investigating the function of NANS using this polyclonal antibody, researchers can gain valuable insights into the underlying mechanisms of disease pathology and potentially identify novel therapeutic strategies.
Product Name:
NANS Rabbit Polyclonal Antibody
SKU:
CAB4778
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-359 of human NANS (NP_061819.2).
This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.
Purification Method:
Affinity purification
Gene ID:
54187
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NANS Rabbit pAb (CAB4778) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
