The NANOS1 Polyclonal Antibody (PACO07274) is a valuable tool for researchers studying NANOS1, a crucial protein involved in germ cell development and maintenance. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it an ideal choice for Western blot applications. By binding specifically to the NANOS1 protein, researchers can accurately detect and analyze its expression in various cell types, facilitating investigations in reproductive biology and developmental genetics.NANOS1 is essential for the regulation of germ cell fate and differentiation, playing a key role in maintaining the pluripotency and self-renewal of germ cells.
Dysregulation of NANOS1 has been associated with infertility and developmental abnormalities, making it a target of interest in reproductive medicine and stem cell research. Understanding the functions of NANOS1 is essential for advancing knowledge in these fields and potentially developing new therapeutic approaches for infertility and other related conditions.
Antibody Name:
NANOS1 Antibody (PACO07274)
Antibody SKU:
PACO07274
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:10000-1:20000, WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from Nanos Homologue 1 (NANOS1) at AA range: 151-200
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200.
Western Blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000.
Synonyms:
Nanos homolog 1 (NOS-1) (EC_Rep1a)
UniProt Protein Function:
NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 10q26.11Cellular Component: perinuclear region of cytoplasm; cytoplasmMolecular Function: protein binding; translation repressor activity; zinc ion binding; RNA bindingBiological Process: cell migration; negative regulation of translationDisease: Spermatogenic Failure 12
UniProt Protein Details:
NCBI Summary:
This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]
