The Nanog Polyclonal Antibody is a powerful tool for researchers studying the Nanog protein, a key transcription factor involved in maintaining pluripotency in embryonic stem cells. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting Nanog in various samples, particularly in Western blot applications. By binding to Nanog, researchers can accurately analyze its expression levels and localization in different cell types, advancing studies in stem cell biology and regenerative medicine.Nanog is a pivotal regulator of stem cell self-renewal and differentiation, making it a critical target for investigations into cell fate determination and tissue regeneration.
Its role in embryonic development and adult tissue maintenance underscores its importance in understanding diseases related to stem cell dysfunction, such as cancer and developmental disorders. By elucidating the signaling pathways and functions of Nanog, researchers can potentially uncover novel therapeutic strategies for regenerative medicine and personalized treatment approaches.
Product Name:
NANOG Polyclonal Antibody
SKU:
CAB22115
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-305 of mouse NANOG (NP_082292.1).
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
71950
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using NANOG antibody (CAB22115) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
