The NAMPT Polyclonal Antibody (PAC064323) is a valuable tool for researchers studying Nicotinamide phosphoribosyltransferase (NAMPT), an enzyme involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in Western blot applications.NAMPT plays a critical role in cellular metabolism and energy regulation, making it a key target for studies in various fields such as immunology, cancer research, and metabolic disorders. Understanding the expression and activity of NAMPT can provide insights into its functions in health and disease, offering potential therapeutic avenues for conditions linked to dysregulated NAD+ metabolism.
With its reliability and specificity, the NAMPT Polyclonal Antibody (PAC064323) is an essential tool for researchers seeking to explore the roles of NAMPT in cellular processes and disease mechanisms. Its ability to detect NAMPT protein in various cell types makes it a versatile asset for experiments aimed at uncovering the molecular mechanisms underlying NAMPT function, ultimately contributing to the development of innovative treatments and interventions.
Western Blot. Positive WB detected in: Rat Heart tissue, Rat Liver tissue, Rat Spleen tissue, Rat Kidney tissue, Rat Brain tissue, Hela whole cell lysate. All lanes: Nampt antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa.
Background:
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
NCBI Summary:
has similarity to cytokines but is not secreted; localized to the nucleus and cytoplasm, changes in subcellular localization may be associated with the cell cycle [RGD, Feb 2006]
