The NAMPT Polyclonal Antibody (CAB0256) is a valuable tool for researchers studying NAMPT, a key enzyme involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the NAMPT protein, this antibody enables researchers to detect and analyze NAMPT expression in various cell types, making it ideal for investigations in metabolism, aging, and cancer research.NAMPT, also known as visfatin, plays a crucial role in maintaining cellular NAD+ levels, which are essential for many biological processes, including energy metabolism, DNA repair, and cell signaling.
Dysregulation of NAMPT has been implicated in several diseases, making it a promising therapeutic target for conditions such as metabolic disorders, neurodegenerative diseases, and certain types of cancer. By understanding the function of NAMPT, researchers can develop novel treatments that modulate NAD+ levels and potentially improve health outcomes for patients with these conditions.
Product Name:
PBEF/NAMPT Rabbit Polyclonal Antibody
SKU:
CAB0256
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 181-382 of human PBEF/Visfatin/NAMPT (NP_005737.1).
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Purification Method:
Affinity purification
Gene ID:
10135
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PBEF/Visfatin/NAMPT Rabbit pAb (CAB0256) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
