The NAGPA Polyclonal Antibody (PACO59804) is a valuable tool for researchers studying N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) and its role in lysosomal storage disorders. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in a variety of experimental techniques, including Western blotting.NAGPA is a key enzyme involved in the breakdown of complex molecules within lysosomes, and its dysfunction has been linked to the development of various lysosomal storage disorders. By targeting NAGPA with this antibody, researchers can investigate its expression levels, localization, and interactions in different cell types, shedding light on the molecular mechanisms underlying these debilitating diseases.
Furthermore, understanding the function of NAGPA is crucial for the development of potential therapies aimed at restoring enzyme activity or targeting alternative pathways to alleviate the symptoms associated with lysosomal storage disorders. The NAGPA Polyclonal Antibody (PACO59804) offers researchers a reliable tool to advance their studies in this important area of biomedicine.
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: NAGPA antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 53, 34 kDa. Observed band size: 53 kDa.
IHC image of PACO59804 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.
Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.
UniProt Protein Details:
NCBI Summary:
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015]
