The NAGK Polyclonal Antibody (CAB9070) is a vital tool for research involving NAGK, an enzyme that plays a key role in the biosynthesis of N-acetyl-L-glutamate (NAG), an important molecule in the urea cycle. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the NAGK protein, this antibody allows for the detection and analysis of NAGK in a variety of cell types, making it ideal for studies in biochemistry and metabolic research.
NAGK is essential for the regulation of the urea cycle, which is responsible for removing ammonia from the body. Dysregulation of NAGK activity has been implicated in various metabolic disorders and diseases, making it a target of interest for researchers studying conditions such as hyperammonemia and urea cycle disorders. Understanding the function of NAGK and its role in metabolic pathways is crucial for the development of targeted therapies and diagnostic tools for these conditions.
Product Name:
NAGK Rabbit Polyclonal Antibody
SKU:
CAB9070
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-210 of human NAGK (NP_060037.3).
This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars.
Purification Method:
Affinity purification
Gene ID:
55577
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NAGK antibody (CAB9070) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
