The NADPH Oxidase 4 Polyclonal Antibody (CAB11274) is a valuable tool for researchers studying NADPH oxidase 4, an enzyme involved in the production of reactive oxygen species (ROS) in various cell types. This antibody, generated in rabbits, is highly specific and reacts strongly with human samples, making it suitable for Western blot applications. By binding to NADPH oxidase 4, this antibody allows for precise detection and analysis, facilitating studies in immunology, oxidative stress, and cardiovascular research.NADPH oxidase 4 is known for its role in ROS production, which can have both beneficial and detrimental effects on cells depending on the context.
Dysregulation of NADPH oxidase 4 has been implicated in various pathological conditions, including cardiovascular diseases, neurodegenerative disorders, and inflammatory conditions. By investigating the function and regulation of NADPH oxidase 4, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
[KO Validated] NOX4 Rabbit Polyclonal Antibody
SKU:
CAB11274
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 328-578 of human NADPH oxidase 4 (NOX4) (NP_058627.1).
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
50507
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (CAB11274) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
