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NAD+/NADH Colorimetric Assay Kit

SKU:
MAES0233
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Animal Tissue, Cell
Sensitivity:
0.02 μmol/L
Range:
0.02-5.0 μmol/L
Assay Time:
70 min
€519
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Description

system_update_altDatasheet
Product Name:NAD+/NADH Colorimetric Assay Kit
Product Code:MAES0233
Sample Type:Animal Tissue, Cell
Assay Time:70 min
Assay Type:Quantitative
Synonyms:NAD+/NADH
Detection Instrument:Microplate reader (450 nm)
Detection Method:Colorimetric method
Sensitivity:0.02 μmol/L
Detection Range:0.02-5.0 μmol/L
Inter-assay CV:0.091
Intra-assay CV:0.018

Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:PBS(0.01 M, pH 7.4)
Other Instruments Required:Micropipettor, 37℃ water bath, 10 KD filters tube

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