NAD+/NADH Colorimetric Assay Kit (MAES0233)
- SKU:
- MAES0233
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Animal Tissue, Cell
- Sensitivity:
- 0.02 μmol/L
- Range:
- 0.02-5.0 μmol/L
- Assay Time:
- 70 min
Description
| Product Name: | NAD+/NADH Colorimetric Assay Kit |
| Product Code: | MAES0233 |
| Sample Type: | Animal Tissue, Cell |
| Assay Time: | 70 min |
| Assay Type: | Quantitative |
| Synonyms: | NAD+/NADH |
| Detection Instrument: | Microplate reader (450 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.02 μmol/L |
| Detection Range: | 0.02-5.0 μmol/L |
| Inter-assay CV: | 0.091 |
| Intra-assay CV: | 0.018 |
Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | PBS(0.01 M, pH 7.4) |
| Other Instruments Required: | Micropipettor, 37℃ water bath, 10 KD filters tube |
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