The NACA Monoclonal Antibody is a cutting-edge tool for researchers studying the NACA protein, an essential component of the ribosome. This antibody, derived from mouse hybridoma cells, offers high specificity and sensitivity in detecting NACA in various experimental settings.NACA is a key player in protein synthesis, serving as a chaperone protein that assists in the correct folding and assembly of newly synthesized proteins. Dysregulation of NACA has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic intervention.
This monoclonal antibody is validated for use in immunofluorescence, immunohistochemistry, and flow cytometry applications, providing researchers with a versatile tool for exploring the functions of NACA in different cellular contexts. By enabling precise detection and visualization of NACA, this antibody opens up new avenues for unraveling the complex mechanisms underlying protein synthesis and its implications for human health.
Product Name:
NACA Monoclonal Antibody
SKU:
CAB22165
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NACA (NP_005585.1).
This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.
Purification Method:
Affinity purification
Gene ID:
4666
Clone Number:
ARC53721
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using NACA antibody (CAB22165) at1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
