The NA NE (Noradrenaline/Norepinephrine) ELISA Kit is a high-quality assay designed for the accurate measurement of noradrenaline and norepinephrine levels in various samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Noradrenaline and norepinephrine are important neurotransmitters and hormones in the body, playing crucial roles in the stress response, mood regulation, and cardiovascular function.
Dysregulation of these catecholamines has been associated with conditions such as depression, anxiety disorders, and hypertension, making them valuable biomarkers for studying these disorders and potential therapeutic interventions.Overall, the NA NE ELISA Kit is a valuable tool for researchers looking to investigate the roles of noradrenaline and norepinephrine in health and disease, providing accurate and precise measurements to support their studies.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
99-112
99-114
Average (%)
96
105
106
1:4
Range (%)
87-102
90-101
95-111
Average (%)
94
96
102
1:8
Range (%)
86-99
90-103
96-110
Average (%)
92
97
102
1:16
Range (%)
92-107
86-98
97-112
Average (%)
97
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
89-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.3
9.5
1.0
2.4
9.6
Standard deviation
0.1
0.1
0.3
0.1
0.1
0.4
C V (%)
9.09
4.35
3.16
10.0
4.17
4.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of NA/NE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes NA/NE in samples. No significant cross-reactivity or interference between NA/NE and analogues was observed.
