The N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody (CAB17924) is an essential tool for researchers studying mRNA modifications and epitranscriptomics. This antibody is specifically designed to target the m6A modification, which plays a crucial role in regulating gene expression and RNA stability.Raised in rabbits, the CAB17924 antibody demonstrates high reactivity with human samples, making it a valuable tool for researchers working in this field. Validated for use in multiple applications, including Western blot and immunofluorescence, this antibody allows for the detection and analysis of m6A-modified RNA in various cell types and tissues.
The m6A modification is known to impact a wide range of biological processes, including RNA processing, translation, and degradation. Dysregulation of m6A modification has been linked to various diseases, such as cancer, neurodevelopmental disorders, and metabolic disorders. By using the CAB17924 antibody, researchers can gain valuable insights into the role of m6A modification in these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
N6-methyladenosine/m6A Rabbit Polyclonal Antibody
SKU:
CAB17924
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Chemical compounds corresponding to N6-methyladenosine / m6A.
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
The m6A rabbit polyclonal antibody (4µg, A17924) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)
