The N6-Methyladenosine (m6A) Monoclonal Antibody is a vital tool for researchers studying RNA methylation, a key modification that regulates gene expression and various cellular processes. This antibody, produced through monoclonal antibody technology, is highly specific to N6-methyladenosine and shows minimal cross-reactivity with other RNA modifications, allowing for reliable and accurate detection in various biological samples.N6-methyladenosine is the most prevalent internal modification in mRNA and long non-coding RNA, playing a crucial role in post-transcriptional gene regulation. Dysregulation of m6A modification has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic conditions.
By targeting and detecting m6A, researchers can gain insights into the mechanisms underlying these diseases and develop potential therapeutic interventions.This monoclonal antibody is suitable for a range of applications, including immunoprecipitation, immunofluorescence, and RNA immunoprecipitation sequencing (RIP-Seq). Its high sensitivity and specificity make it an essential tool for scientists studying RNA methylation and its implications in health and disease. By utilizing this antibody, researchers can advance their understanding of m6A biology and contribute to the development of novel treatments targeting RNA modification pathways.
Product Name:
N6-methyladenosine / m6A Monoclonal Antibody
SKU:
CAB22411
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Chemical compounds corresponding to N6-methyladenosine / m6A.
N6-methyladenosine / m6A, U-2 OS treated with UV(30s)Brdu(10min) cells
Observed MW:
Refer to figures
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
Purification Method:
Affinity purification
Gene ID:
CAS:1867-73-8
Clone Number:
ARC5003-03
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,0.05% BSA,50% glycerol,pH7.3.
The N6-methyladenosine / m6A Rabbit mAb (4µg,A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin)Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin)
