Myosin heavy chain Rabbit Monoclonal Antibody (CAB4963)
The Myosin Heavy Chain Rabbit Monoclonal Antibody (CAB4963) is a specialized antibody designed for research involving myosin heavy chain, a protein essential for muscle contraction and movement. This antibody, developed in rabbits, is highly specific and reactive towards myosin heavy chain present in human samples, making it an excellent tool for studying muscle physiology and function.Myosin heavy chain is a critical component of muscle fibers, contributing to their structure and contractile activity. Research on this protein is essential for understanding muscle development, function, and diseases such as muscular dystrophy and heart failure. The Myosin Heavy Chain Rabbit Monoclonal Antibody enables the detection and analysis of myosin heavy chain in various cell types, making it invaluable for studies in physiology, pharmacology, and muscle-related disorders.
With its high specificity and sensitivity, this antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Its ability to bind to myosin heavy chain with precision allows for accurate detection and quantification, facilitating detailed investigations into muscle structure and function. Researchers in the fields of biology, medicine, and biomechanics will find this antibody indispensable for their studies on muscle biology and related disorders.
Product Name:
Myosin heavy chain Rabbit Monoclonal Antibody
SKU:
CAB4963
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1400 of human Myosin heavy chain (P12883).
CMD1S; CMH1; MPD1; MYHCB; SPMD; SPMM; Myosin heavy chain
Positive Sample:
Mouse lung,Mouse skeletal muscle,Rat heart
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, myofibril.
Calculated MW:
224kDa
Observed MW:
250kDa
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
4625/4624
Clone Number:
ARC1170
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Myosin heavy chain Rabbit mAb (CAB4963) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
