Anti-MYOG Antibody (CAB17427)
- SKU:
- CAB17427
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
Anti-MYOG Antibody (CAB17427)
The MYOG Polyclonal Antibody (CAB17427) is a valuable tool for researchers studying MYOG, a key transcription factor involved in muscle differentiation and development. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards MYOG in human samples, making it an ideal choice for Western blot applications.MYOG plays a crucial role in regulating the expression of muscle-specific genes and promoting muscle cell differentiation. Dysregulation of MYOG activity has been linked to muscle-related disorders and diseases, making it a target of interest in the fields of muscle biology and therapeutics.
By utilizing the MYOG Polyclonal Antibody, researchers can detect and analyze MYOG protein levels in various cell types, furthering our understanding of its function in muscle development and physiology. This antibody is a valuable resource for studies focusing on muscle differentiation, regeneration, and disease mechanisms, offering insights that may lead to the development of novel therapeutic strategies for muscle-related conditions.
| Antibody Name: | Anti-MYOG Antibody |
| Antibody SKU: | CAB17427 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-224 of human MYOG (NP_002470.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-224 of human MYOG (NP_002470.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELY ETSP YFYQ EPRF YDGE NYLP VHLQ GFEP PGYE RTEL TLSP EAPG PLED KGLG TPEH CPGQ CLPW ACKV CKRK SVSV DRRR AATL REKR RLKK VNEA FEAL KRST LLNP NQRL PKVE ILRS AIQY IERL QALL SSLN QEER DLRY RGGG GPQP GVPS ECSS HSAS CSPE WGSA LEFS ANPG DHLL TADP TDAH NLHS LTSI VDSI TVED VSVA FPDE TMPN |
| Gene ID: | 4656 |
| Uniprot: | P15173 |
| Cellular Location: | Nucleus |
| Calculated MW: | 25kDa |
| Observed MW: | 35kDa |
| Synonyms: | MYOG, MYF4, bHLHc3, myf-4, myogenin, Myogenin |
| Background: | Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. |
| UniProt Protein Function: | myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: nucleoplasm; nucleus; transcription factor complex Molecular Function:chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity Biological Process: mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; negative regulation of cell proliferation; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle development; striated muscle atrophy |
| NCBI Summary: | Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P15173 |
| NCBI GenInfo Identifier: | 127625 |
| NCBI Gene ID: | 4656 |
| NCBI Accession: | P15173.2 |
| UniProt Secondary Accession: | P15173,Q53XW6, |
| UniProt Related Accession: | P15173 |
| Molecular Weight: | 25,037 Da |
| NCBI Full Name: | Myogenin |
| NCBI Synonym Full Names: | myogenin |
| NCBI Official Symbol: | MYOGÂ Â |
| NCBI Official Synonym Symbols: | MYF4; myf-4; bHLHc3Â Â |
| NCBI Protein Information: | myogenin |
| UniProt Protein Name: | Myogenin |
| UniProt Synonym Protein Names: | Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4 |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYOGÂ Â |
 | Western blot analysis of extracts of Rat skeletal muscle, using MYOG Rabbit pAb (CAB17427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of NIH/3T3 cells using MYOG antibody (CAB17427) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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