The MyoF Polyclonal Antibody (CAB15427) is a crucial tool for researchers studying MyoF, a key protein involved in controlling muscle contraction and movement. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting MyoF in human samples, making it an invaluable asset for Western blot analyses.MyoF, also known as myogenic factor, plays a pivotal role in skeletal muscle development and function by regulating the expression of genes involved in muscle growth and differentiation. Dysregulation of MyoF has been linked to muscle disorders and neuromuscular diseases, making it an attractive target for therapeutic interventions aimed at treating these conditions.
By enabling the precise detection and quantification of MyoF protein levels in various cell types, the MyoF Polyclonal Antibody facilitates in-depth investigations into the molecular mechanisms underlying muscle physiology and pathology. Researchers can utilize this antibody to explore the role of MyoF in muscle diseases, exercise physiology, and regenerative medicine, ultimately advancing our understanding of muscle biology and paving the way for innovative therapeutic strategies.
Product Name:
MYOF Rabbit Polyclonal Antibody
SKU:
CAB15427
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MYOF (NP_038479.1).
Cell membrane, Cytoplasmic vesicle membrane, Nucleus membrane, Single-pass type II membrane protein.
Calculated MW:
235kDa
Observed MW:
250kDa
Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined.
Purification Method:
Affinity purification
Gene ID:
26509
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and MYOF knockout (KO) HeLa(KO) cells, using [KO Validated] MYOF Rabbit pAb (CAB15427) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
